T4 polynucleotide kinaseLieferant: EuroClone
EK0032EA 207 EUREK0032 EK0031
T4 polynucleotide kinase
Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).
- Detection of DNA modification by the [³²P]-postlabelling assay
- 5'-phosphorylation of oligonucleotides, PCR products, and DNA or RNA prior to ligation
- Removal of 3'-phosphoryl groups
- Phosphorylation of PCR primers
T4 polynucleotide kinase exhibits 5'-phosphatase activity and catalyses the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction). The enzyme is also a 3'-phosphatase.
Labelling 5'-termini of nucleic acids to be used as probes for hybridisation, probes for transcript mapping, markers for gel electrophoresis, primers for DNA sequencing, primers for PCR.
Bitte beachten For research use only. Not for use in diagnostic procedures.
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