Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells. Humsn histone H2B is a 125 amino acid protein.
Histone H2B antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.
Protein G Column
PBS, 0.02% sodium azide.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Reactivity: Bovine, Chicken, Drosophila, Human, Insect, Mouse, Orangutan, Rat, Zebrafish