RAMP3 is a member of the RAMP family of single-transmembrane-domain proteins, called receptor-activity-modifying proteins. RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. Co-expression of RAMP3 with CRLR results in the production of a functional adrenomedullin receptor.
RAMP3 antibody can be used in ELISA starting at 1:3000 - 1:6000, Western Blot starting at 1:3000 - 1:6000, immunohistochemistry starting at 10 μg/mL, and immunoprecipitation
PBS, 0.01% sodium azide, 50% glycerol.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.