Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.
SAFB antibody can be used in Western Blot starting at 1:1000 - 1:3000, and immunohistochemistry starting at 10 μg/mL.
Protein G Column
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Reactivity: Gibbon, Gorilla, Human, Monkey, Orangutan