There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100 kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97 kDa (-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.
DNA Ligase III antibody can be used in ELISA, Western Blot, and immunohistochemistry starting at 5 μg/mL.
Protein G Column
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.