NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.
Nucleophosmin antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 10 μg/mL, immunofluorescence, immunoprecipitation, and flow cytometry starting at 1:25 - 1:50.
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol. BSA is present at 1 mg/mL final concentration (USA origin).
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.