IKKalpha, an IkappaB-type protein kinase, is a member of the multimeric IkappaB kinase complex. This complex, often consisting of IKKalpha/IKKbeta heterodimers plus regulatory IKKgamma subunits, phosphorylates IkappaB proteins, marking them for destruction and thereby allowing activation of NFkappaB and downstream nuclear genes. The constitutively expressed IKKalpha protein contains a N-terminal kinase domain, a central leucine zipper motif, and a C-terminal helix-loop-helix motif. In knockout studies, IKKalpha (-/-) mice died perinatally and displayed a range of developmental defects; thus, IKKalpha is believed to function in development, particularly in development of skin keratinocyte, cornea, and conjunctiva.
CHUK antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:100, and immunofluorescence starting at 1:200 - 1:1000.
PBS, 0.02% sodium azide, 50% glycerol.
Aliquot and store at -80°C. Avoid multiple freeze-thaw cycles.