CDC25A, a CDC25 dual specificity protein phosphatase, has been shown to activate the cyclin-dependent kinases at different points of the cell cycle. CDC25A has been shown to affect S phase entry and possibly the initiation of mitosis.CDC25A dephosphorylates cyclin dependent kinases, including Cdk2, Cdk4, as well as non cyclin proteins such as the homeodomain transcription factor cut, the proto-oncogene Raf-1. CDC25A has been implicated in many carcinomas, including those of the colon, lymph node, stomach, ovary, breast. CDC25A is located on chromosome 3p21 in an area frequently involved in karyotypic abnormalities in renal carcinomas, small cell carcinomas of the lung, and benign tumors of the salivary gland.
CDC25A antibody can be used in ELISA, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:100, immunofluorescence starting at 1:200 - 1:1000, and flow cytometry starting at 1:200 - 1:400.
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.