Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy-terminal region of the protein. Akt/PKB phosphorylation is critical for it activity.
AKT3 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:200, immunofluorescence starting at 1:200 - 1:1000, and flow cytometry starting at 1:200 - 1:400.
Protein G Column
PBS, 0.05% BSA, 0.05% sodium azide.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Bat, Bovine, Chicken, Dog, Gibbon, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat