NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.
NEK2 antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 10 μg/mL.
0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, 0.01% sodium azide.
Store NEK2 antibody at -20 °C prior to opening. Dilute only prior to immediate use. Avoid multiple freeze-thaw cycles.