The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified.
CD8A antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation
PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Aliquot and store at 4°C. Avoid freezing.