ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 100 kDa band observed in human brain (amygdala and cerebral cortex) lysates (calculated MW of 90.5 kDa according to NP_055585.1). Recommended concentration: 0.3-1 μg/mL.
PIKE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PIKE antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.