ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 48 kDa band observed in HeLa lysate (calculated MW of 47.4 kDa according to NP_001983.2). Recommended concentration: 0.5-2 μg/mL.
PAR1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PAR1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.