ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 30 kDa band observed in human bone marrow lysates (calculated MW of 31.5 kDa according to NP_005212.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, J Biol Chem. 2003 Sep 5;278 (36) :34387-94; PMID: 12815054). Recommended concentration: 1-3 μg/mL.
DLX5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DLX5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.