ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 70 kDa band observed in mouse and rat liver lysates (calculated MW of 68.7 kDa according to human NP_001477.1 and of 68.9 kDa according to rat NP_037252.1). Recommended concentration: 0.1-0.3 μg/mL.
GCKR antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GCKR antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Mouse, Rat