ELISA: Antibody detection limit dilution 1:64000. Approximately 24 kDa band observed in human lung lysates (calculated MW of 23.0 kDa according to NP_005333.2). Recommended concentration: 0.01-0.03 μg/mL.
HMGB3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HMGB3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.