ELISA: Antibody detection limit dilution 1:16000. Approximately 25 kDa band observed in rat brain lysates (calculated MW of 25.2 kDa according to rat NP_742030.1 (Evectin-1) and of 27.2 kDa according to human NP_067023.1). Recommended concentration: 1-3 μg/mL.
PHR1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
pH R1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.