ELISA: Antibody detection limit dilution 1:32000. Preliminary experiments in lysates of K562 gave no specific signal but low background (at antibody concentration up to 1 μg/mL).
homeobox B4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
homeobox B4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.