ELISA: Antibody detection limit dilution 1:32000. Approximately 60 kDa band in human brain (cerebral cortex and frontal cortex) lysates after 0.3 μg/mL antibody staining.1 and of 136 kDa according to NP_001120703.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide.
AUTS2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
AUTS2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.