ELISA: Antibody detection limit dilution 1:1000. Preliminary experiments in human prostate lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL).
PSCA antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PSCA antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.