ELISA: Antibody detection limit dilution 1:8000. Approximately 60 kDa band observed in lysates of A549 and HeLa (calculated MW of 63.5 kDa according to NP_002078.1). Recommended concentration: 1-3 μg/mL.
Granulin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Granulin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.