ELISA: Antibody detection limit dilution 1:32000. Approximately 37 kDa band observed in human kidney lysates (calculated MW of 38.4 kDa according to NP_002139.2). Recommended concentration: 0.2-0.6 μg/mL. Additional bands of unknown identity were also consistently observed at 90+26 kDa . These bands were successfully blocked by incubation with the immunizing peptide.
HOXD10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HOXD10 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.