ELISA: Antibody detection limit dilution 1:128000. Approximately 85 kDa band observed in human brain (Substantia nigra and amygdala) lysates (calculated MW of 84.6 kDa according to NP_848605.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band of unknown identity was also consistently observed at 35 kDa . This band was successfully blocked by incubation with the immunizing peptide.
ANKK1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ANKK1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.