ELISA: Antibody detection limit dilution 1:8000. Approximately 28 kDa band in lysates of cell line NIH3T3 after 1 μg/mL antibody staining. The 28 kDa band was successfully blocked by incubation with the immunizing peptide.
TRPM8 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TRPM8 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.