ELISA: Antibody detection limit dilution 1:16000. Approximately 75 kDa band observed in K562 lysate (calculated MW of 74.4 kDa according to NP_062552.2). Recommended concentration: 0.3-1 μg/mL.
BIN3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
BIN3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.