ELISA: Antibody detection limit dilution 1:16000. Experiments gave bands at approximately 150 kDa and 50 kDa in human skeletal Mucle lysate after 0.5 μg/mL antibody staining. This protein has a calculated MW of 76.0 kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 μg/mL.
TRC8 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TRC8 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.