ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 110 kDa band observed in rat brain lysates (calculated MW of 112 kDa according to human NP_057263.1 and to rat NP_536321.1). Recommended concentration: 0.3-1 μg/mL.
TRPC4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TRPC4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.