ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 55 kDa band observed in human lung lysates (calculated MW of 52.9 kDa according to NP_009093.1). Recommended concentration: 1-3 μg/mL.
Transcription Factor EB antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Transcription Factor EB antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.