ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 90 kDa band observed in human skin lysates (calculated MW of 99.3 kDa according to NP_001008536.1). Recommended concentration: 1-3 μg/mL.
Trichohyalin-like 1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Trichohyalin-like 1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.