ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately110 kDa band observed in human skeletal muscle lysates (calculated MW of 109 kDa according to NP_055573.2). Recommended concentration: 0.3-1 μg/mL.
Sorting Nexin 19 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Sorting Nexin 19 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.