ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 30 kDa band observed in Jurkat lysate (calculated MW of 21.7 kDa according to NP_005082.1). The observed molecular weight corresponds to earlier findings in liter Immunohistochemistry: In paraffin embedded human spleen shows staining of select splenocytes. Recommended concentration, 3-6 μg/mL.
PGLYRP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PGLYRP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.