ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Preliminary experiments in human liver, heart and ovary lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feed
Palladin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Palladin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.