ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 23 kDa band in human colon, heart and ovary lysates after 0.1 μg/mL antibody staining. Immunohistochemistry: In paraffin embedded human thyroid gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration, 2-5 μg/mL.
Frizzled 2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Frizzled 2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.