ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 14 kDa band observed in lysates of cell line A431 (calculated MW of 16.6 kDa according to NP_002643.1). Recommended concentration: 0.3-1 μg/mL.
EAT2 Phospho Y127 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
EAT2 pH ospH o Y127 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.