ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 65 kDa band observed in mouse brain lysates (calculated MW of 68.1 kDa according to mouse NP_084122.2). Recommended concentration: 0.01-0.1 μg/mL.
ATG16L1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ATG16L1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.