ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 35 kDa band observed in human liver lysates (calculated MW of 35.4 kDa according to NP_001629.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows staniing of droplets in the cytoplasm of hepatocytes. Recommended concentration, 2.5-3.8 μg/mL.
Apolipoprotein F antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Apolipoprotein F antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.