ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Bands corresponding to the exopected sizes of ApoB100 and ApoB48 were detected in human serum samples from five different individuals. Recommended concentration: 0.3-1 μg/mL.
Apolipoprotein B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Apolipoprotein B antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.