ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 50-55 kDa band in human brain (cerebellum, cerebral cortex, frontal cortex), heart, skeletal muscle and testis lysates after 0.3 μg/mL.
ABCA8 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ABCA8 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.