ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 35 kDa band observed in human lysates of cell lines A431 and K562 (calculated MW of 38.7 kDa according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al, tumor Res. 2000 Nov15;60 (22) :6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 μg/mL.
Annexin A1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Annexin A1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat