ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 70 kDa band observed in human brain (cerebellum and frontal cortex) lysates (calculated MW of 61.6 kDa according to NP_000800.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cortex shows patchy staining near or at the neuronal cell membrane. Recommended concentration, 2-4 μg/mL.
GABRA4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GABRA4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.