ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 38 kDa band observed in human cerebellum, mouse brain and rat brain lysates. Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cerebellum shows pixulate staining in the cells of the granular layer. Recommended concentration, 2.5-3.8 μg/mL.
EPM2A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
EPM2A antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat