ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 80 kDa band observed in nuclear HeLa lysate (calculated MW of 74.8 kDa according to NP_001131.3). Recommended concentration: 0.2-0.5 μg/mL.
ALOX15 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ALOX15 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.