ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 50-55 kDa band observed in human liver and human placenta lysates (calculated MW of 32.7 kDa according to NP_116179.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al,,J Biol Chem. 2007 Oct 5;282 (40) :29448-56.; PMID: 17702753). Recommended concentration: 0.03-0.1 μg/mL.
ORAI1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ORAI1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.