ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 60 kDa band observed in lysates of Daudi (calculated MW of 63.0 kDa according to NP_055413.1). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 28 kDa . This band was successfully blocked by incubation with the immunizing peptide.
SOCS7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SOCS7 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.