ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 40 kDa band observed in lysates of cell lines DAUDI and MOLT4 (calculated MW of 44.3 kDa according to NP_663615.1). Recommended concentration: 1-3 μg/mL.
APOL3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
APOL3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.