ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 28 kDa band observed in mouse spleen lysates (calculated MW of 28.2 kDa according to human NP_001635.2 and 27.5 kDa acccording to mouse NP_112436.1). Recommended concentration:1-3 μg/mL.
APOBEC1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
APOBEC1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.