ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 45 kDa band observed in nuclear HeLa lysate (calculated MW of 50.7 kDa according to NP_001779.3 and of 55.6 kDa according to NP_001011553.2). Recommended concentration: 0.3-1 μg/mL.
SEPT7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SEPT7 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.