ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 23 to 24 kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 22.1 kDa according to NP_859048.1). Recommended concentration: 0.01-0.03 μg/mL.
Prdx1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Prdx1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.