ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 80 kDa band in human spleen lysates after 0.2 μg/mL antibody staining.3 kDa according to NP_002931.2. The ~80 kDa band was successfully blocked by incubation with the immunizing peptide.
RNase L antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RNase L antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.