ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 60 kDa band observed in human pancreas lysates (calculated MW of 58.0 kDa according to NP_036066.2). Recommended concentration: 0.03-0.1 μg/mL.
FTO antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FTO antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.